DNA laddering

Jump to: navigation, search

DNA laddering is a phenomenon seen in laboratory tests; it is a sensitive indicator of programmed cell death, specifically of apoptosis.

Endonuclease activation is a characteristic feature of apoptosis. This degrades genomic DNA at internucleosomal linker regions and produces 180- to 185- base-pair DNA fragments. On agarose gel electrophoresis, these give a characteristic "laddered" appearance. The dying cell's morphological changes are short-lived and difficult to detect. DNA laddering has therefore become a sensitive method to distinguish apoptosis from ischemic or toxic cell death.[1]

Citations

  • M Iwata, D Myerson, B Torok-Storb and RA Zager (1996). "An evaluation of renal tubular DNA laddering in response to oxygen deprivation and oxidant injury". Retrieved 17 April. Unknown parameter |accessyear= ignored (|access-date= suggested) (help); Check date values in: |accessdate= (help)



Linked-in.jpg