Cholesterol oxidase

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In enzymology, a cholesterol oxidase (EC is an enzyme that catalyzes the chemical reaction

cholesterol + O2 cholest-4-en-3-one + H2O2

Thus, the two substrates of this enzyme are cholesterol and O2, whereas its two products are cholest-4-en-3-one and H2O2.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with oxygen as acceptor. The systematic name of this enzyme class is cholesterol:oxygen oxidoreductase. Other names in common use include cholesterol- O2 oxidoreductase, 3beta-hydroxy steroid oxidoreductase, and 3beta-hydroxysteroid:oxygen oxidoreductase. This enzyme participates in bile acid biosynthesis.

Structural studies

As of late 2007, 14 structures have been solved for this class of enzymes, with PDB accession codes 1B4V, 1B8S, 1CBO, 1CC2, 1COY, 1I19, 1IJH, 1MXT, 1N1P, 1N4U, 1N4V, 1N4W, 2GEW, and 3COX.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
  • PubMed Central references for
  • Google Scholar references for
  • Richmond W (1973). "Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum". Clin. Chem. 19: 1350&ndash, 6. PMID 4757363.
  • STADTMAN TC, CHERKES A, ANFINSEN CB (1954). "Studies on the microbiological degradation of cholesterol". J. Biol. Chem. 206: 511&ndash, 23. PMID 13143010.

External links

The CAS registry number for this enzyme class is 9028-76-6.

Gene Ontology (GO) codes