C5orf3 (chromosome 5open reading frame 3) is a gene on chromosome 5 in humans that encodes a protein FAM114A2. This protein has a function that is not well known. C5orf3 is, however, highly conserved in mammals with homologs both in fungi and plants.
The c5orf3 protein is 505 amino acids long[1] with a molecular weight of 55.5 kdal and an isoelectric point of 4.66.[2] It is predicted to stay in the nucleus after translation [3]
There is evidence that c5orf3 interacts with another protein of unknown function from chromosome 5, c5orf4 [4]
This protein is thought to include a P loop[5] that suggests a role in ATP- and/or GTP-binding [6]
Gene
The c5orf3 gene is located on chromosome 5 (5q31-33).[7] This gene has 14 exons spanning through its sequence.[1] The coding sequence is 2886 base pairs with a 5’ UTR of 94 base pairs and a 3’ UTR of 1273 base pairs.[1] It is expressed at high levels in most tissues of the human body.[7] It is also highly expressed in tissues in the human brain [8]
↑Saraste M, Sibbald PR, Wittinghofer A (November 1990). "The P-loop--a common motif in ATP- and GTP-binding proteins". Trends Biochem. Sci. 15 (11): 430–4. doi:10.1016/0968-0004(90)90281-f. PMID2126155.
Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID14702039.
Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID9373149.
Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID8125298.
