ARHGEF4

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Identifiers
Aliases
External IDs	GeneCards: [1]
Orthologs
Species	Human	Mouse
Entrez	n/a	n/a
Ensembl	n/a	n/a
UniProt	n/a	n/a
RefSeq (mRNA)	n/a	n/a
RefSeq (protein)	n/a	n/a
Location (UCSC)	n/a	n/a
PubMed search	n/a	n/a
Wikidata
View/Edit Human

Rho guanine nucleotide exchange factor 4 is a protein that in humans is encoded by the ARHGEF4 gene.

Function

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein-coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. This protein is similar to rat collybistin protein. Alternative splicing of this gene generates two transcript variants that encode different isoforms. Also, there is possibility for the usage of multiple polyadenylation sites for this gene.

Model organisms

Arhgef4 knockout mouse phenotype

Characteristic	Phenotype
Homozygote viability	Normal
Fertility	Normal
Body weight	Normal
Anxiety	Normal
Neurological assessment	Normal
Grip strength	Normal
Hot plate	Normal
Dysmorphology	Normal
Indirect calorimetry	Normal
Glucose tolerance test	Normal
Auditory brainstem response	Normal
DEXA	Normal
Radiography	Normal
Body temperature	Normal
Eye morphology	Normal
Clinical chemistry	Normal
Haematology	Normal
Peripheral blood lymphocytes	Abnormal
Micronucleus test	Normal
Heart weight	Normal
Salmonella infection	Normal
Citrobacter infection	Normal
All tests and analysis from

Model organisms have been used in the study of ARHGEF4 function. A conditional knockout mouse line, called Arhgef4^{tm1a(KOMP)Wtsi} was generated as part of the International Knockout Mouse Consortium program — a high-throughput mutagenesis project to generate and distribute animal models of disease to interested scientists.

Male and female animals underwent a standardized phenotypic screen to determine the effects of deletion. Twenty two tests were carried out on homozygous mutant mice and one significant abnormality was observed: males has atypical peripheral blood lymphocyte parameters, including a decreased B cell number and an increased granulocyte number.

Interactions

ARHGEF4 has been shown to interact with APC.

External links

• Human ARHGEF4 genome location and ARHGEF4 gene details page in the UCSC Genome Browser.

Further reading

• Nakajima D, Okazaki N, Yamakawa H, et al. (2003). "Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones". DNA Res. 9 (3): 99–106. doi:10.1093/dnares/9.3.99. PMID 12168954.
• Kuraguchi M, Wang XP, Bronson RT, et al. (2006). "Adenomatous polyposis coli (APC) is required for normal development of skin and thymus". PLoS Genet. 2 (9): e146. doi:10.1371/journal.pgen.0020146. PMC 1564426. PMID 17002498.
• Watanabe T, Wang S, Noritake J, et al. (2005). "Interaction with IQGAP1 links APC to Rac1, Cdc42, and actin filaments during cell polarization and migration". Dev. Cell. 7 (6): 871–83. doi:10.1016/j.devcel.2004.10.017. PMID 15572129.
• Kawasaki Y, Sato R, Akiyama T (2003). "Mutated APC and Asef are involved in the migration of colorectal tumour cells". Nat. Cell Biol. 5 (3): 211–5. doi:10.1038/ncb937. PMID 12598901.
• Kawasaki Y, Senda T, Ishidate T, et al. (2000). "Asef, a link between the tumor suppressor APC and G-protein signaling". Science. 289 (5482): 1194–7. doi:10.1126/science.289.5482.1194. PMID 10947987.