3-hydroxybutyrate dehydrogenase

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In enzymology, a 3-hydroxybutyrate dehydrogenase (EC is an enzyme that catalyzes the chemical reaction

(R)-3-hydroxybutanoate + NAD+ acetoacetate + NADH + H+

Thus, the two substrates of this enzyme are (R)-3-hydroxybutanoate and NAD+, whereas its 3 products are acetoacetate, NADH, and H+.

This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. The systematic name of this enzyme class is (R)-3-hydroxybutanoate:NAD+ oxidoreductase. Other names in common use include NAD+-beta-hydroxybutyrate dehydrogenase, hydroxybutyrate oxidoreductase, beta-hydroxybutyrate dehydrogenase, D-beta-hydroxybutyrate dehydrogenase, D-3-hydroxybutyrate dehydrogenase, D-(-)-3-hydroxybutyrate dehydrogenase, beta-hydroxybutyric acid dehydrogenase, 3-D-hydroxybutyrate dehydrogenase, and beta-hydroxybutyric dehydrogenase. This enzyme participates in synthesis and degradation of ketone bodies and butanoate metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1WMB and 1X1T.


  • IUBMB entry for
  • BRENDA references for (Recommended.)
  • PubMed references for
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  • Google Scholar references for
  • Bergmeyer HU, Gawehn K, Klotzsch H, Krebs HA, Williamson DH (1967). "Purification and properties of crystalline 3-hydroxybutyrate dehydrogenase from Rhodopseudomonas spheroides". Biochem. J. 102: 423&ndash, 31. PMID 4291491.
  • Delafield FP, Cooksey KE, Doudoroff M (1965). "beta-Hydroxybutyric dehydrogenase and dimer hydrolase of Pseudomonas lemoignei". J. Biol. Chem. 240: 4023&ndash, 8. PMID 4954074.
  • Lehninger AL, Sudduth HC and Wise JB (1960). "D-beta-Hydroxybutyric dehydrogenase of mitochondria". J. Biol. Chem. 235: 2450&ndash, 2455.

External links

The CAS registry number for this enzyme class is 9028-38-0.

Gene Ontology (GO) codes